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BIOPHEN™ Heparin (AT+)
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BIOPHEN™ Heparin (AT+) (221007) is a measurement of Heparin, and Heparin-like anticoagulants, using an antiXa kinetics chromogenic method, with addition of exogenous ATIII FOR RESEARCH USE ONLY. Manufactured by HYPHEN BioMed.
Biophen Heparin (AT+) is a kinetics chromogenic anti-Xa method developed for measuring homogeneously heparin (UFH) and Low Molecular Weight Heparin (LMWH), using the same calibration curve, provided that the adaptation used allows this superimposition. Heparin is a sulphated polysaccharide with a high affinity for antithrombin. When complexed with heparin, antithrombin exhibits a fast acting and potent inhibitory activity for coagulant serine esterases: IXa, Xa and thrombin. LMWH, and heparin analogues, such as Sodium Danaparoïd, inhibit more efficiently Factor Xa than thrombin. Anti-Xa assays are then the methods of choice for measuring heparins and their analogues. Biophen Heparin (AT+) is a kinetics method based on the inhibition of a constant amount of factor Xa, by the tested heparin in presence of exogenous antithrombin, and hydrolysis of a Factor Xa specific chromogenic substrate (SXa-11), by the factor Xa in excess. pNA is then released from the substrate. The amount of pNA released is then a relation of the residual factor Xa activity. There is an inverse relationship between the concentration of heparin and color development, measured at 405 nm.