Check-1 FABP Qualitative 10mins

Heart-type fatty acid-binding protein (H-FABP or FABP) is a small cytosolic protein that is abundant in cardiac tissue. After myocardialischemic damage, H-FABP can be detected in the blood as early as within 1 hour after onset of chest pain, with peak values reached at 3–6 hours and plasma levels returning to normal within 24–30 hours (1). The combination of initial H-FABP release after symptom onset, rapid kidney clearance from the circulation, and high cardiac specificity makes it as an early marker for the diagnosis of AMI (2,3). The H-FABP can also be used to detect perioperative AMIs and detect re infarction if it occurs within 10 hours after symptom onset (4).

FABP-CHECK-1 is a diagnostic rapid test kit to be used if the occurrence of an acute myocardial infarction (AMI) is suspected. The test kit can be used between 30 minutes and 6 hours after onset of symptoms. FABP levels return to base line within 24 hours.

FABP-CHECK-1 is a rapid quantitative assay for the measurement of Heart-type fatty acid-binding protein in serum, plasma or whole blood samples. The method employs a unique combination of two different monoclonal dye conjugate and monoclonal solid phase antibodies to identify FABP in the test samples with a high degree of sensitivity. As the test sample flows through the absorbent device, the antibody-dye conjugate binds to the h-FABP forming an antibody-antigen complex.

This complex binds to the anti FABP antibody in the reaction zone (T) and produces a pink-rose color band when FABP concentration is sufficient. The reaction mixture continues flowing through the absorbent device past the reactive zone and control zone (C). Unbound conjugate binds to the reagents in the control zone (C), producing a pink-rose color band, demonstrating that the reagents are functioning correctly.