Mouse Anti-2019 nCoV(S)IgM ELISA Kit
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Mouse Anti-2019 nCoV(S)IgM ELISA Kit (EM1908) allows for the in vitro quantitative determination of anti-2019 nCoV (S) IgM concentrations in serum, plasma, cell culture supernatant and other biological samples. Manufactured by FineTest.
This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with anti-2019 nCoV (S) IgM bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-2019 nCoV (S) IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
