Strongyloides Ratti ELISA Kit
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The Bordier Strongyloides Ratti ELISA Kit (9450) is an enzyme immunoassay for the diagnosis of human strongyloidiasis. Manufactured by Bordier Affinity Products.
Intended Use:
The Bordier Strongyloides ratti ELISA kit is intended for the quantitative detection of IgG antibodies against Strongyloides nematodes in human serum. Serology is an aid for diagnosis and cannot be used as the sole method of diagnosis.
Background:
Strongyloidiasis is caused by the nematode Strongyloides stercoralis. Humans can be infected by contacting contaminated soil with Strongyloides larvae, which can percutaneously enter the body through exposed skin, such as bare feet. Most infected people do not show any symptoms, or they exhibit unspecific signs such as stomachache, nausea, diarrhea, cough or rash. However, in some cases, hyperinfection syndrome and disseminated strongyloidiasis can occur in people who are on corticosteroids, other immunosuppressive therapies or who are suffering from AIDS or other immunodeficiencies. Diagnosis is based on detection of parasite larvae in stool and a positive result by serological testing.
Principle and Presentation:
The kit provides all the material needed to perform 96 enzyme-linked immunosorbent assays (ELISA) on breakable microtitration wells sensitized with Strongyloides ratti somatic larval antigens. Specific antibodies in the sample will bind to these antigens and washing will remove unspecific antibodies. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate. A second washing step will remove unbound conjugate. Revealing bound antibodies is made by the addition of pNPP substrate which turns yellow in the presence of alkaline phosphatase. Color intensity is proportional to the amount of Strongyloides ratti specific antibodies in the sample. Potassium phosphate is added to stop the reaction. Absorbance at 405 nm is read using an ELISA microplate reader.
The test can be performed with automatic systems, but this must be validated by the user.
Product Specific Publications:
- Bisoffi Z, Buonfrate D, Sequi M, et al. Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. PLoS Negl Trop Dis. 2014;8(1):e2640. doi:10.1371/journal.pntd.0002640
- Buonfrate D, Sequi M, Mejia R, et al. Accuracy of five serologic tests for the follow up of Strongyloides stercoralis infection. PLoS Negl Trop Dis. 2015;9(2):e0003491. doi:10.1371/journal.pntd.0003491
- Autier B, Boukthir S, Degeilh B, et al. Clinical value of serology for the diagnosis of strongyloidiasis in travelers and migrants: A 4-year retrospective study using the Bordier IVD® Strongyloides ratti ELISA assay. Parasite. 2021;28:79. doi:10.1051/parasite/2021075
- Lu Y, Ramachandran V, Mahanty S, et al. Comparison of Strongyloides-specific IgG enzyme immunoassays for laboratory detection of Strongyloides stercoralis infection. Pathology. 2022;54(1):127-129. doi:10.1016/j.pathol.2021.03.002
- Tamarozzi F, Guevara ÁG, Anselmi M, et al. Accuracy, acceptability, and feasibility of diagnostic tests for the screening of Strongyloides stercoralis in the field (ESTRELLA): a cross-sectional study in Ecuador. Lancet Glob Health. 2023;11(5):e740-e748. doi:10.1016/S2214-109X(23)00108-0
Other Relevant Publications:
- Formenti F, Buonfrate D, Prandi R, et al. Comparison of S. stercoralis Serology Performed on Dried Blood Spots and on Conventional Serum Samples. Front Microbiol. 2016;7:1778. doi:10.3389/fmicb.2016.01778
- Diep NTN, Thai PQ, Trang NNM, et al. Strongyloides stercoralis seroprevalence in Vietnam. Epidemiol Infect. 2017;145(15):3214-3218. doi:10.1017/S0950268817002333
- Are Bordier kits CE-marked?
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Yes. The Bordier kits are CE-marked for Medical Professional Use only in accordance with EU IVD regulations (IVDD 98/79/EC).
- Do Bordier hold ISO accreditation?
- Which components can be used with all Bordier ELISA kits?
- Can Bordier ELISA kits be automated?
- Is it be possible to order single components?
- Can Bordier ELISA kits be used with plasma?
- Can Bordier ELISA kits be used with animal serum?
- Can Bordier ELISA kits be used with other samples (cerebrospinal fluid, bronchoalveolar lavage, aqueous humor…)?
- Is a positive control provided with IFAT kits?
